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Calculate DNA base percentages (A = T, C = G)

12th grade (Year 13 in England) · 16 questions · Common Core: NGSS.HS-LS3-1, NGSS.SEP 5 · England: GCSE Biology: DNA structure, A level Biology: Nucleic acids

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  1. Question 1

    Why does DNA with a high percentage of C and G need more energy to separate its strands?

    • A. C–G DNA is single-stranded
    • B. A and T are not paired
    • C. C and G are heavier atoms
    • D. C–G pairs are held by three hydrogen bonds, A–T pairs by two
  2. Question 2

    A double-stranded DNA fragment has 1000 bases in total. 350 of them are cytosine. How many are adenine?

    Answer

  3. Question 3

    In a sample of double-stranded DNA, 30 % of the bases are thymine (T). What percentage are cytosine (C)?

    Answer%

  4. Question 4

    Chargaff found A ≈ T and C ≈ G in many species. Which model did this support?

    • A. DNA as a single straight strand
    • B. complementary base pairing in the double helix
    • C. a triple helix
    • D. proteins carrying genetic information
  5. Question 5

    A double-stranded DNA fragment has 600 bases in total. 120 of them are cytosine. How many are adenine?

    Answer

  6. Question 6

    In a sample of double-stranded DNA, 26 % of the bases are adenine (A). What percentage are guanine (G)?

    Answer%

  7. Question 7

    A sample has 21 % A, 29 % T, 25 % C and 25 % G. What does this suggest?

    • A. It is normal double-stranded DNA
    • B. The measurements prove an error in all DNA
    • C. It is likely single-stranded DNA, because A ≠ T
    • D. It must be RNA because it contains T
  8. Question 8

    A double-stranded DNA fragment has 400 bases in total. 80 of them are cytosine. How many are adenine?

    Answer

  9. Question 9

    In a sample of double-stranded DNA, 15 % of the bases are thymine (T). What percentage are cytosine (C)?

    Answer%

  10. Question 10

    Chargaff found A ≈ T and C ≈ G in many species. Which model did this support?

    • A. complementary base pairing in the double helix
    • B. proteins carrying genetic information
    • C. a triple helix
    • D. DNA as a single straight strand
  11. Question 11

    A double-stranded DNA fragment has 1000 bases in total. 200 of them are cytosine. How many are adenine?

    Answer

  12. Question 12

    In a sample of double-stranded DNA, 15 % of the bases are adenine (A). What percentage are guanine (G)?

    Answer%

  13. Question 13

    Chargaff found A ≈ T and C ≈ G in many species. Which model did this support?

    • A. a triple helix
    • B. DNA as a single straight strand
    • C. complementary base pairing in the double helix
    • D. proteins carrying genetic information
  14. Question 14

    A double-stranded DNA fragment has 600 bases in total. 120 of them are cytosine. How many are adenine?

    Answer

  15. Question 15

    In a sample of double-stranded DNA, 20 % of the bases are adenine (A). What percentage are guanine (G)?

    Answer%

  16. Question 16

    A sample has 21 % A, 29 % T, 25 % C and 25 % G. What does this suggest?

    • A. The measurements prove an error in all DNA
    • B. It is likely single-stranded DNA, because A ≠ T
    • C. It must be RNA because it contains T
    • D. It is normal double-stranded DNA

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Answer key: Calculate DNA base percentages (A = T, C = G)

  1. 1. D. C–G pairs are held by three hydrogen bonds, A–T pairs by two

  2. 2. 150

  3. 3. 20 %

  4. 4. B. complementary base pairing in the double helix

  5. 5. 180

  6. 6. 24 %

  7. 7. C. It is likely single-stranded DNA, because A ≠ T

  8. 8. 120

  9. 9. 35 %

  10. 10. A. complementary base pairing in the double helix

  11. 11. 300

  12. 12. 35 %

  13. 13. C. complementary base pairing in the double helix

  14. 14. 180

  15. 15. 30 %

  16. 16. B. It is likely single-stranded DNA, because A ≠ T