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Science lesson plan · 9th grade (Year 10 in England) · 60 minutes

Calculate and use Rf values in chromatography

Materials and their properties

CCSS NGSS.HS-PS1-3CCSS NGSS.SEP 5England: GCSE Combined Science: Chemical analysis (required practical: chromatography)

Objective and success criteria

Students will: Calculate and use Rf values in chromatography.

  • I can answer questions on today's skill on my own, without help.
  • I can explain each step and say why it works.
  • I can check my answer and spot a mistake.

Before and after

Needs first: Pure substances, mixtures and chromatography

Leads to: the next skill in this area.

Materials: A tray of everyday objects, Sorting hoops, Mini whiteboards, A diagram or short video clip of the idea, Printed student sheet (below).

Warm-up · 6 min

  • Quick retrieval from earlier learning (Pure substances, mixtures and chromatography). Students answer on mini whiteboards.
  • Show answers together and note who needs support in the main task.
  1. In a chromatogram the solvent moved 10 cm and a dye spot moved 5 cm from the start line. What is the Rf value of the dye? (Give 2 decimal places.) → 0.5Prompt: Rf = distance moved by the spot ÷ distance moved by the solvent
  2. How can you test whether a sample of water is pure? (Check that it boils at exactly 100 °C (at sea-level pressure) / Check that it looks clear / Shake it / Smell it) → Check that it boils at exactly 100 °C (at sea-level pressure)Prompt: Pure substances have a fixed melting point and boiling point.
  3. Purity and chromatography: which statement is NOT true? (A pure substance has a fixed melting point. / An Rf value can be greater than 1. / Mixtures can be separated by physical methods. / In chromatography, a more soluble dye usually travels further.) → An Rf value can be greater than 1.Prompt: Think about what 'pure' means in chemistry.

Hook · 6 min

  • Pass round objects made of different materials and ask why each one was chosen.
  • Share the objective: Calculate and use Rf values in chromatography. Read the success criteria together.

I do: model it · 12 min

  • Work through the example out loud, saying why you do each step, not just what you do.
  • Example: On a chromatogram the solvent front moved 12 cm from the start line and a spot moved 0.1 cm. What is the Rf value of the spot? Give 2 decimal places.
  • Step 1: 0.1 ÷ 12 = 0.008
  • Step 2: So the answer is: 0.01
  • Answer: 0.01
  • Pause after each step and ask a student to say what comes next.

We do: guided practice · 12 min

  • Do these together. Students try each step on whiteboards before you reveal it.
  • Use the hints as prompts rather than giving the answer.
  1. On a chromatogram the solvent front moved 5 cm from the start line and a spot moved 0.5 cm. What is the Rf value of the spot? Give 2 decimal places. → 0.1Prompt: Rf = distance moved by the spot ÷ distance moved by the solvent
  2. A dye has an Rf value of 0.5 in water. If the solvent front moves 8 cm, how far will the dye move, in cm? → 4 cmPrompt: Rearrange Rf = spot distance ÷ solvent distance.

You do: independent practice · 17 min

  • Students work on the student sheet. Circulate and check the first two answers of every student early.
  • Students who finish: write a question of their own for a partner, with the answer.

The 8 questions are on the student sheet below, with answers in the key.

Exit ticket · 7 min

  • Two questions on their own. Sort answers into got it / nearly / not yet to plan the next lesson.
  1. Reference Rf values in the same solvent: dye A 0.15, dye B 0.35, dye C 0.55, dye D 0.8. An unknown ink gives one spot that moves 1.75 cm when the solvent moves 5 cm. Which dye is it? (dye D / dye C / dye A / dye B) → dye BPrompt: Calculate the Rf of the unknown spot.
  2. On a chromatogram the solvent front moved 5 cm from the start line and a spot moved 3.3 cm. What is the Rf value of the spot? Give 2 decimal places. → 0.66Prompt: Rf = distance moved by the spot ÷ distance moved by the solvent

Watch out for

  • Everyday meanings of words getting in the way of the science meaning: define key words explicitly.
  • Remembering facts without the reason: ask "why?" and "what is the evidence?"

Support and stretch

Support

  • Review first: Pure substances, mixtures and chromatography.
  • Use concrete materials: a tray of everyday objects.
  • Give the first step of each question, or a partly worked example to finish.
  • Pair with a confident partner for the guided practice.

Stretch

  • Explain a common mistake in writing and how to fix it.
  • Write a word problem that uses today's skill, then solve a partner's.

Homework and more practice

Free online practice with hints and printable worksheets for this skill: eurekami.com/science/grade-9.

Free lesson plan from eurekami.com/lesson-plan-builder

eurekami

Calculate and use Rf values in chromatography

Name

Date

Score/ 8

  1. Question 1

    Reference Rf values in the same solvent: dye A 0.15, dye B 0.35, dye C 0.55, dye D 0.8. An unknown ink gives one spot that moves 4 cm when the solvent moves 5 cm. Which dye is it?

    • A. dye A
    • B. dye B
    • C. dye D
    • D. dye C
  2. Question 2

    A dye has an Rf value of 0.2 in water. If the solvent front moves 4 cm, how far will the dye move, in cm?

    Answercm

  3. Question 3

    On a chromatogram the solvent front moved 12 cm from the start line and a spot moved 2.5 cm. What is the Rf value of the spot? Give 2 decimal places.

    Answer

  4. Question 4

    A dye has an Rf value of 0.3 in water. If the solvent front moves 8 cm, how far will the dye move, in cm?

    Answercm

  5. Question 5

    On a chromatogram the solvent front moved 6 cm from the start line and a spot moved 0.8 cm. What is the Rf value of the spot? Give 2 decimal places.

    Answer

  6. Question 6

    Reference Rf values in the same solvent: dye A 0.15, dye B 0.35, dye C 0.55, dye D 0.8. An unknown ink gives one spot that moves 0.75 cm when the solvent moves 5 cm. Which dye is it?

    • A. dye C
    • B. dye A
    • C. dye B
    • D. dye D
  7. Question 7

    A dye has an Rf value of 0.45 in water. If the solvent front moves 8 cm, how far will the dye move, in cm?

    Answercm

  8. Question 8

    On a chromatogram the solvent front moved 5 cm from the start line and a spot moved 2.8 cm. What is the Rf value of the spot? Give 2 decimal places.

    Answer

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Answer key: Calculate and use Rf values in chromatography

  1. 1. C. dye D

  2. 2. 0.8 cm

  3. 3. 0.21

  4. 4. 2.4 cm

  5. 5. 0.13

  6. 6. B. dye A

  7. 7. 3.6 cm

  8. 8. 0.56